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[주간세미나] 2015년 9월 21일
관리자 2015-09-16

<권대혁교수 Lab.>

* 발표자 : 신종혁

* 제목 :  Enhanced Production of Lipophilic Molecules through the Formation of Caveola-like Structures inside Escherichia coli

* 내용 : Caveolae are membrane-budding structures that exist in many vertebrate cells. One of the important functions of caveolae is to form membrane curvature (caveolae) and endocytic vesicles (endosome). Caveolae-like structures are formed in Escherichia coli through the expression of caveolin-1. We hypothesized that the caveolae-like sturcture might expand membrane surface area of E. coli, providing more storage space for lipophilic products and membrane bound-proteins. Enlarged membrane surface area of E. coli provided tolerance for toxic fatty acid and augmented expression of membrane protein. The biotransformation of ricinoleic acid into ester by an alcohol dehydrogenase (ADH) and Baeyer-Villiger monooxygenase (BVMO) was utilized as the model system. The recombinant E.coli co-expressing ADH, BVMO and caveolin-1 showed durable bioconversion activity and ~80% improved bioconversion activity. Collection of lipophilic products (ester) in the caveolae-like structures is expected to improve the production of lipophilic products in E. coli.

* 발표자 : 박준범

* 제목 : Chemical modification of flavonoid for improved Physicochemical properties and bioavailibility

* 내용 : Phenolic compounds are secondary metabolites produced in plants by the shikimic acid pathway. It has already been demonstrated that phenolic compounds have a wide range of bioactivities including antioxidative, anticarcinogenic, anti-inflammatory properties which are mostly related to the potential health-promoting benefits against human health risks such as hypertension, obesity, cardiovascular diseases, diabetes and cancer. Furthermore, phenolic compounds are multi-active components used in common cosmetics primarily for antioxidant and soothing actions. However, some phenolic compounds have poor solvent solubility, weak stability to pH and light, too strong colors to apply for cosmetics. Here, we chemically modified a model flavonoid. Long chain fatty acids were attached to the flavonoid using 1-(3-Dimethylaminopropyl)3-ethylcarbodiimide (EDC) and 4-Dimethylaminopyridine (DMAP). In addition, oxalyl chloride and fatty acids were reacted to phenolic compounds with pyridine base . These phenolic derivatives were characterized using GC/MS and 1H NMR. Compared to native phenolic compounds, novel compound JB20 showed improved characteristics. Cosmetic and therapeutic effects of JB20 are currently under investigation.
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